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KMID : 1101420200520020112
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Korean Journal of Clinical Laboratory Science
2020 Volume.52 No. 2 p.112 ~ p.118
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Histological Examination of Engineered Mesenchymal Stem Cells Improve Bladder Function in Rat
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Cho Eun-Kyung
Jeon Seung-Hwan
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Abstract
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This study was undertaken to examine the effects and to investigate the relevant mechanisms of overexpressing stromal cell-derived factor-1 (SDF-1) produced by engineered mesenchymal stem cells, in a neurogenic bladder (NB) rat model. Sprague-Dawley (SD) rats (N=48) were randomly divided into 4 groups comprising 12 rats each: control group, Injury group, Injury+imMSC group, and Injury+SDF-1 eMSC group. Rats in the Injury+imMSC group were treated with imMSCs, whereas the Injury+SDF-1 eMSC group were administered SDF-1 eMSCs. After 4-weeks therapy, the bladder and pelvic nerve (PN) tissues were examined by subjecting to Masson’s trichrome staining and immunofluorescence. Administration of SDF-1 eMSC resulted in improved smooth muscle content in the bladder tissue, significantly increased β-III tubulin expression of the PN, and enhanced SDF-1 expression (P＜0.05). The bladder wall repair can be attributed to the overexpression of SDF-1 by SDF-1 eMSCs. Significantly increased SDF-1 expression was obtained in the Injury+SDF-1 eMSC group (P＜0.05). The crushed PN also showed significant recovery in the Injury+SDF-1 eMSC group (P＜0.05). In conclusion, our results indicate that SDF-1 eMSCs express more SDF-1 in vivo, thereby facilitating the repair of injured nerve and recovery of NB in rats.
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KEYWORD
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Immortalized mesenchymal stem cells, Neurogenic bladder, SDF-1 engineered mesenchymal stem cells
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